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Micropropagation
Plant Tissue Culture Research
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Hibiscus Tissue Culture: From Micropropagation to 8-Inch Blooms

As part of my ongoing tissue culture research, I focused last year on producing a large quantity of Hibiscus through micropropagation. Using sterile lab techniques and established protocols, I was able to generate a consistent batch of healthy Hibiscus clones, ideal for further experimentation and observation.

After rooting, the plantlets went through careful acclimation—a crucial step for any tissue-cultured specimen. Transitioning them from the controlled, sterile lab environment to ambient conditions required a gradual adjustment in humidity, airflow, and light. Once hardened off, a subset was selected for overwintering under hydroponic grow lights.

The indoor lighting setup allowed me to maintain active growth throughout the colder months, simulating optimal light conditions despite the UK’s limited winter sunlight. This period not only helped the plants develop stronger stems and foliage but also provided valuable insight into how tissue-cultured Hibiscus respond to hydroponic lighting compared to natural seasonal changes.

With spring’s arrival, all the plants were moved into my conservatory, where they had access to natural light and a warmer environment. The transition was seamless, thanks to their prior acclimation and the continuous care provided over winter. Now, as the temperatures rise, the reward is evident—many of these Hibiscus are coming into flower with stunning 8-inch blooms.

Hibiscus Bloom

The results are visually impressive and reinforce the potential of tissue culture not just for propagation, but also for producing robust, ornamental plants capable of thriving and flowering on schedule. These large blooms are a testament to the quality and consistency that plant tissue culture can deliver when paired with thoughtful post-lab care.

This Hibiscus project is one of many experiments documented here on micropropagation.co.uk. As always, my aim is to explore the practical applications of plant tissue culture—from lab protocols to real-world growing conditions—and share results that inspire fellow plant enthusiasts, growers, and researchers alike. Stay tuned for more updates as I continue testing new species and refining methods in my home tissue culture lab.

Published 28th May 2025

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